RESOURCES

Name of Method: Thermofluor

Result expected:
Can screen for complex formation - starting from individual components, complex formation can be followed
Can screen conditions that stabilize complexes - starting from the complex, buffer systems and/or additional additives that stabilize complexes could be identified.

KD range: N/A

MW range: generally applicable

Purity: Preferably crystallization level.

Labeling-Tagging: Should any or all of reactants be labeled or tagged and how ?
N/A

Accuracy: (Da)
N/A

Quantity required: For a complex AB, assuming that A is the 'receptor' and B the 'ligand' how much protein is needed to characterize the complex fully (e.g. obtain KD or get MW) ? Doesn't measure KD or MW - screens ligands and stabilizing factors.
Approximately 10?g protein per experiment

HTP: Is the method HTP (can it use 96-well sample plates in an automated fashion)?
Yes - 96 well format

Speed: Will the user have a quantitative answer immediately after collecting the data?
Yes shift in the melting curve

Cost: (approximate cost of consumables per ONE experiment)
Varies between ?10-50.

Access: Is an instrument available (name all SPINE2 sites you know)?
Oxford, Marseille, Stockholm, Amsterdam, Florence, Weizmann, Hamburg

User expertise: Can the method be provided as a service (measurement and data analysis done by local staff) or only access and assistance on a collaborative basis?
Access and assistance on a collaborative basis

Special requirements: Any special sample needs?
Concentrate to ~0.5 mg/ml.