Timetable of Tasks, Deliverables and Milestones for the first 18 months:

8.2 Planning and timetable - Gantt chart showing the timed plan for each workpackage, with deliverables and milestones

Task Description 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
WP1.1 Complexes in the ubiquitination pathway                                    
1.1.1 Identification and characterisation of targets                                    
1.1.2 Co-expression screening of components                                    
1.1.3 Optimisation of complexes +/- target and ubiquitin                                    
1.1.4 Scale-up and QA of first set of suitable samples                                    
1.1.5 Screen crystallization and NMR of first complexes                                    
1.1.6 Progress first complexes into structure determination                                    
D1.1.1 Construct design and expression data for protein domains                                    
D1.1.2 Expression data for complexes                                    
D1.1.3 Expression and QA for protein domains                                    
D1.1.4 Biophysical data for complexes                                    
M1.1.1 Expression of complexes                                    
M1.1.2 First purified protein domains                                    
M1.1.3 First set of stable complexes into crystn and NMR screens                                    

  ACTIVITY PERIOD   TASK ACTIVITY   DELIVERABLES DUE   MILESTONE

 

Task Description 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
WP1.2 Cell life, death and integrity                                    
1.2.1 Co-expression screening in E.coli of protein complexes                                    
1.2.2 Expression screening of complexes in eukaryotic systems                                    
1.2.3 Use of truncation libraries to identify soluble domains                                    
1.2.4 Scale-up and QA of suitable samples                                    
1.2.5 Assess assembly and stability of complexes biophysically                                    
1.2.6 Screen crystn properties and NMR spectra                                    
1.2.7 Progress first targets into structure determination                                    
D1.2.1 Expression data for components and complexes                                    
D1.2.2 Biophysical data for complexes and components                                    
M1.2.1 Expression of some components and complexes                                    
M1.2.2 Characterisation of first components and complexes                                    
M1.2.3 Start crystallization trials and NMR screening                                    

  ACTIVITY PERIOD   TASK ACTIVITY   DELIVERABLES DUE   MILESTONE

 

Task Description 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
WP1.3 Complexes in development and synaptic signalling                                    
1.3.1 Co-expression screening in E.coli of protein complexes                                    
1.3.2 Expression screening of complexes in eukaryotic systems                                    
1.3.3 Co-expression screening of problematic molecules                                    
1.3.4 Use of truncation libraries for soluble domains                                    
1.3.5 Scale-up, 15N and 13C labelling and QA of samples                                    
1.3.6 Analyse intrinsically disordered cytoplasmic domains                                    
1.3.7 Screen crystn and NMR samples in first targets                                    
1.3.8 Progress first targets into structure determination                                    
D1.3.1 Expression data for some components and complexes                                    
D1.3.2 Biophysical data for first set of complexes                                    
M1.3.1 Purification and labelled samples of first proteins                                    
M1.3.2 Characterisation of first samples and X-ray/NMR screens                                    

  ACTIVITY PERIOD   TASK ACTIVITY   DELIVERABLES DUE   MILESTONE

 

Task Description 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
WP1.4 Protein kinases                                    
1.4.1 Co-expression screening of protein kinases and modulators                                    
1.4.2 Expression screening of IFNR extracellular domains                                    
1.4.3 Co-expression screening of  IFNR extracellular domains                                    
1.4.4 Scale-up and QA of suitable samples                                    
1.4.5 Assess assembly and stability of complexes biophysically                                    
1.4.6 Screen cyrstn properties, SAXS and NMR spectra                                    
1.4.7 Progress first targets into structure determination                                    
D1.4.1 Expression data for some components and complexes                                    
D1.4.2 Biophysical data for first set of complexes                                    
M1.4.1 Expression of some components and complexes                                    
M1.4.2 Characterisation of first samples and X-ray/NMR screens                                    

  ACTIVITY PERIOD   TASK ACTIVITY   DELIVERABLES DUE   MILESTONE

 

Task Description 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
WP1.5 Protein phosphatases                                    
1.5.1 Multi-expression screening of protein phosphatases                                    
1.5.2 Scale-up and QA of suitable samples                                    
1.5.3 Assess assembly and stability of complexes biophysically                                    
1.5.4 Screen crystallization properties of targets                                    
1.5.5 Progress first targets into structure determination                                    
D1.5.1 Expression data for some components and complexes                                    
D1.5.2 Biophysical data for first set of complexes                                    
M1.5.1 Expression of some components and complexes                                    
M1.5.2 Characterisation of first samples and X-ray/NMR screens                                    

  ACTIVITY PERIOD   TASK ACTIVITY   DELIVERABLES DUE   MILESTONE

 

Task Description 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
WP1.6 Transcription complexes                                    
1.6.1 Identn and charactn of target NR and TF assemblies                                    
1.6.2 Co-expression screening of components and complexes                                    
1.6.3 Scale-up and QA of first set of components and complexes                                    
1.6.4 Biophysical charactn of assemblies and complex stability                                    
1.6.5 Screen crystallization properties of first products                                    
1.6.6 Progress first products into structure determination                                    
D1.6.1 Expression data for first products                                    
D1.6.2 Biophysical data for first products                                    
M1.6.1 Expression of some components and complexes                                    
M1.6.2 Characterisation of first samples and X-ray/NMR screens                                    

  ACTIVITY PERIOD   TASK ACTIVITY   DELIVERABLES DUE   MILESTONE

 

Task Description 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
WP1.7 Innate immune system                                    
1.7.1 Selection of complexes involved in pathogen recognition                                    
1.7.2 Selection, expression and optimisation of complexes                                    
1.7.3 Purifn, crystn and structure determination of complexes                                    
D1.7.1 Cloning and sequence verification of targets                                    
D1.7.2 Expression of >1target and assess expression/solubility                                    
D1.7.3 Crystallization trails of >1 target protein                                    
M1.7.1 Cloning of targets                                    
M1.7.2 Analysis of expression/ identify optimal route for solubility                                    
M1.7.3 Obtain a soluble expressed protein                                    

  ACTIVITY PERIOD   TASK ACTIVITY   DELIVERABLES DUE   MILESTONE

 

Task Description 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
WP1.8 Adaptive immune system                                    
1.8.1 Expression screening of components in E. coli                                    
1.8.2 Expression screening of components in eukaryotic systems                                    
1.8.3 Identification and production of additional ligands                                    
1.8.4 Co-expression of any cytoplasmic targets                                    
1.8.5 Scale-up and QA of suitable samples                                    
1.8.6 Characterise recognition/binding properties of receptors                                    
1.8.7 Screen crystallization properties                                    
1.8.8 Progress first targets into structure determination                                    
D1.8.1 Expression data for some TCR, NKR & WAFL protein                                    
D1.8.2 Biophysical data for TCR, NKR & WAFL protein                                    
M1.8.1 Expression data for some TCR, NKR & WAFL protein                                    
M1.8.2 Biophysical charactn of binding properties of receptors                                    

  ACTIVITY PERIOD   TASK ACTIVITY   DELIVERABLES DUE   MILESTONE

 

Task Description 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
WP1.9 Viral subversion of cell signals & immune modulation                                    
1.9.1 Identn and charactn of target viral proteins and partners                                    
1.9.2 Co-expression screening of components and complexes                                    
1.9.3 Optimisation of stability for viral proteins with partners                                    
1.9.4 Scale-up & biophysical chractn of first suitable samples                                    
1.9.5 Screen crystallization properties of first viral products                                    
1.9.6 Progress first viral products into structure determination                                    
D1.9.1 Expression data for first viral proteins                                    
D1.9.2 Biophysical data for first viral proteins and complexes                                    
M1.9.1 Expression of viral proteins/complexes                                    
M1.9.2 First set of viral complexes into crystn and NMR screens                                    

  ACTIVITY PERIOD   TASK ACTIVITY   DELIVERABLES DUE   MILESTONE