Technologies
- Development of suitable vectors for co-expression of protein complexes
- Use of prokaryotic and eukaryotic expression systems for the production of complexes
- Refolding of proteins produced as inclusion bodies
- Benchmarking of the Alvinella thermotolerant ortholog strategy using proteins suggested by all partners
- Investigation of C. diabolis model system
- Single protein mutagenesis and screening
- Multi-protein mutagenesis and screening (coexpression)
- Screening and selection procedures for co-crystallization chaperones
- Biophysical characterisation methods for complex formation and stabilisation
- Complex-directed crystallization protocols
- In vivo characterisation of complexes
- Hybrid methods for complex structure determination
- HTP X-ray screening and data collection fro microcrystals
- Diffraction enhancement by controlled dehydration at a synchrotron
- Interface mapping and complex structure determination
- Methods for paramagnetic protein complexes
- Protein complex docking procedures
- Extension of data model for handling protein complexes
- Provision of a central target tracking and simple LIMS system for partners
- Integration of bioinformatics tools into a stand-alone platform available to partners
SPINE2-COMPLEXES is funded by the European Commission FP
